منابع مشابه
Maize (Zea mays L.).
Agrobacterium tumefaciens-mediated transformation is an effective method for introducing genes into maize. In this chapter, we describe a detailed protocol for genetic transformation of the maize genotype Hi II. Our starting plant material is immature embryos cocultivated with an Agrobacterium strain carrying a standard binary vector. In addition to step-by-step laboratory transformation proced...
متن کاملB chromosomes in popcorn (Zea mays L.).
Cytological analysis of microsporogenesis in 72 popcorn plants, comprising nine from the original population (CMS-43, S(0)) and 63 from seven cycles of self-fertilization (S(1) to S(7)), one plant of S(0) generation (plant 2) was identified with B chromosomes. The number of B chromosomes varied from two to three in the same anther. The pattern of chromosome pairing and meiotic behavior of Bs we...
متن کاملIn Vitro Gibberellin A(1) Binding in Zea mays L.
The first and second leaf sheaths of Zea mays L. cv Golden Jubilee were extracted and the extract centrifuged at 100,000g to yield a supernatant or cytosol fraction. Binding of [(3)H]gibberellin A(1) (GA(1)) to a soluble macromolecular component present in the cytosol was demonstrated at 4 degrees C by Sephadex G-200 chromatography. The binding component was of high molecular weight (HMW) and g...
متن کاملGenotyping Analysis of Six Maize (zea Mays L.) Hybrids Using Dna Fingerprinting Technology
The objective of the present study was to conduct DNA based genotyping analysis for hybrid identification in maize (Zea mays L.) using DNA fingerprinting technology. To achieve this random amplified polymorphic DNA (RAPD) analysis was employed. A total of 40 random tenmer primers were surveyed. Of these, OPR-03, OPR-11 and OPT-06 were polymorphic which not only verified the purity among three l...
متن کاملPhytoremediation Potential of Maize (Zea mays L.). A Review
In most developing parts of the world, the rapid expansion of industrial areas leading to uncontrolled processing and utilization of industrial products, agricultural inputs, vehicular emissions and improper disposal of liquid and solid wastes may cause soil to become contaminated with chemical hazards such as heavy metals. Heavy metal contamination of soil may pose risks to humans and the ecos...
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ژورنال
عنوان ژورنال: New Phytologist
سال: 1942
ISSN: 0028-646X,1469-8137
DOI: 10.1111/j.1469-8137.1942.tb07068.x